› 2nsm

X-ray diffraction
2.1Å resolution

Crystal structure of the human carboxypeptidase N (Kininase I) catalytic domain

Released:
DOI: 10.2210/pdb2nsm/pdb
PDB entry 2nsm coloured by chain, front view.
PDB entry 2nsm coloured by chain, side view.
PDB entry 2nsm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the human carboxypeptidase N (kininase I) catalytic domain.
Keil C, Maskos K, Than M, Hoopes JT, Huber R, Tan F, Deddish PA, Erdös EG, Skidgel RA, Bode W
J Mol Biol 366 504-16 (2007)
PMID: 17157876

Function and Biology Details

Reaction catalysed: 
Release of a C-terminal basic amino acid, preferentially lysine.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxypeptidase N catalytic chain Chain: A
Molecule details ›
Chain: A
Length: 439 amino acids
Theoretical weight: 50.18 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P15169 (Residues: 21-458; Coverage: 100%)
Gene names: ACBP, CPN1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NAG 3 x NAG
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P3121
Unit cell:
a: 150.03Å b: 150.03Å c: 54.91Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.179 0.201
Expression system: Komagataella pastoris

Quick links

Citations

7 review citations

Angiotensin I-converting enzyme inhibitors are allosteric enhancers of kinin B1 and B2 receptor function.
Erdös et al. (2010)
6 more

6 mentions without citation

Effects of Glycosylation on the Enzymatic Activity and Mechanisms of Proteases.
Goettig P. (2016)
5 more