› 2nun

X-ray diffraction
2.4Å resolution

The structure of the type III effector AvrB complexed with ADP

Released:
DOI: 10.2210/pdb2nun/pdb
PDB entry 2nun coloured by chain, front view.
PDB entry 2nun coloured by chain, side view.
PDB entry 2nun coloured by chain, top view.
Source organism: Pseudomonas savastanoi pv. glycinea
Primary publication:
Type III effector activation via nucleotide binding, phosphorylation, and host target interaction.
Desveaux D, Singer AU, Wu AJ, McNulty BC, Musselwhite L, Nimchuk Z, Sondek J, Dangl JL
PLoS Pathog 3 e48 (2007)
PMID: 17397263

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Avirulence protein B Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 36.18 KDa
Source organism: Pseudomonas savastanoi pv. glycinea
Expression system: Escherichia coli
UniProt:
  • Canonical: P13835 (Residues: 1-321; Coverage: 100%)
Gene name: avrB
Sequence domains: Avirulence protein
Structure domains: Fic-like fold

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P65
Unit cell:
a: 122.717Å b: 122.717Å c: 64.091Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.233 0.232 0.254
Expression system: Escherichia coli

Quick links

Citations

27 review citations

Susceptibility genes 101: how to be a good host.
van Schie et al. (2014)
26 more

4 mentions without citation

Fido, a novel AMPylation domain common to fic, doc, and AvrB.
Kinch et al. (2009)
3 more