› 2nw7

X-ray diffraction
2.7Å resolution

Crystal Structure of Tryptophan 2,3-dioxygenase (TDO) from Xanthomonas campestris in complex with ferric heme. Northeast Structural Genomics Target XcR13

Released:
DOI: 10.2210/pdb2nw7/pdb
PDB entry 2nw7 coloured by chain, front view.
PDB entry 2nw7 coloured by chain, side view.
PDB entry 2nw7 coloured by chain, top view.
Source organism: Xanthomonas campestris pv. campestris
Primary publication:
Molecular insights into substrate recognition and catalysis by tryptophan 2,3-dioxygenase.
Forouhar F, Anderson JL, Mowat CG, Vorobiev SM, Hussain A, Abashidze M, Bruckmann C, Thackray SJ, Seetharaman J, Tucker T, Xiao R, Ma LC, Zhao L, Acton TB, Montelione GT, Chapman SK, Tong L
Proc Natl Acad Sci U S A 104 473-8 (2007)
PMID: 17197414

Function and Biology Details

Reaction catalysed: 
L-tryptophan + O(2) = N-formyl-L-kynurenine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185742 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan 2,3-dioxygenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 306 amino acids
Theoretical weight: 35.73 KDa
Source organism: Xanthomonas campestris pv. campestris
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8PDA8 (Residues: 1-298; Coverage: 100%)
Gene names: XCC0432, kynA
Sequence domains: Tryptophan 2,3-dioxygenase
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments


Cofactor: Ligand HEM 4 x HEM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 88.939Å b: 109.359Å c: 125.513Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.257 0.257 0.263
Expression system: Escherichia coli

Quick links

Citations

27 review citations

Heme enzyme structure and function.
Poulos TL. (2014)
26 more

3 mentions without citation

Molecular insights into substrate recognition and catalysis by tryptophan 2,3-dioxygenase.
Forouhar et al. (2007)
2 more