› 2nyb

X-ray diffraction
1.1Å resolution

Crystal structure of E.Coli Iron Superoxide Dismutase Q69E at 1.1 Angstrom resolution

Released:
DOI: 10.2210/pdb2nyb/pdb
PDB entry 2nyb coloured by chain, front view.
PDB entry 2nyb coloured by chain, side view.
PDB entry 2nyb coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
How can a single second sphere amino acid substitution cause reduction midpoint potential changes of hundreds of millivolts?
Yikilmaz E, Porta J, Grove LE, Vahedi-Faridi A, Bronshteyn Y, Brunold TC, Borgstahl GE, Miller AF
J Am Chem Soc 129 9927-40 (2007)
PMID: 17628062

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142805 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase [Fe] Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 192 amino acids
Theoretical weight: 21.16 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AGD3 (Residues: 2-193; Coverage: 100%)
Gene names: JW1648, b1656, sodB
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand FE2 4 x FE2
Ligand O 4 x O
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 43.508Å b: 107.596Å c: 84.12Å
α: 90° β: 94.89° γ: 90°
R-values:
R R work R free
0.16 0.16 0.174
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Proton-coupled electron transfer.
Huynh et al. (2007)
5 more

4 mentions without citation

Superoxide dismutases and superoxide reductases.
Sheng et al. (2014)
3 more