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› 2nz2

X-ray diffraction
2.4Å resolution

Crystal structure of human argininosuccinate synthase in complex with aspartate and citrulline

Released:
DOI: 10.2210/pdb2nz2/pdb
Model geometry
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PDB entry 2nz2 coloured by chain, front view.
PDB entry 2nz2 coloured by chain, side view.
PDB entry 2nz2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human argininosuccinate synthetase.
Karlberg T, Collins R, van den Berg S, Flores A, Hammarström M, Högbom M, Holmberg Schiavone L, Uppenberg J
Acta Crystallogr D Biol Crystallogr 64 279-86 (2008)
PMID: 18323623

Function and Biology Details

Reaction catalysed: 
L-citrulline + L-aspartate + ATP = 2-(N(omega)-L-arginino)succinate +AMP + diphosphate + H(+).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133741 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Argininosuccinate synthase Chain: A
Molecule details ›
Chain: A
Length: 413 amino acids
Theoretical weight: 46.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00966 (Residues: 1-412; Coverage: 100%)
Gene names: ASS, ASS1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NA 1 x NA
Ligand ASP 1 x ASP
Ligand CIR 1 x CIR
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: F222
Unit cell:
a: 95.94Å b: 117.47Å c: 155.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.194 0.265
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

Arginine deprivation and argininosuccinate synthetase expression in the treatment of cancer.
Delage et al. (2010)
5 more

6 mentions without citation

Plumbagin elicits differential proteomic responses mainly involving cell cycle, apoptosis, autophagy, and epithelial-to-mesenchymal transition pathways in human prostate cancer PC-3 and DU145 cells.
Qiu et al. (2015)
5 more