› 2o02

X-ray diffraction
1.5Å resolution

Phosphorylation independent interactions between 14-3-3 and Exoenzyme S: from structure to pathogenesis

Released:
DOI: 10.2210/pdb2o02/pdb
PDB entry 2o02 coloured by chain, front view.
PDB entry 2o02 coloured by chain, side view.
PDB entry 2o02 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Phosphorylation-independent interaction between 14-3-3 and exoenzyme S: from structure to pathogenesis.
Ottmann C, Yasmin L, Weyand M, Veesenmeyer JL, Diaz MH, Palmer RH, Francis MS, Hauser AR, Wittinghofer A, Hallberg B
EMBO J 26 902-13 (2007)
PMID: 17235285

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158973 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein zeta/delta Chains: A, B
Molecule details ›
Chains: A, B
Length: 230 amino acids
Theoretical weight: 26.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63104 (Residues: 1-230; Coverage: 94%)
Gene name: YWHAZ
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
ExoS (416-430) peptide Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 14 amino acids
Theoretical weight: 1.37 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand BEZ 2 x BEZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 71.33Å b: 72.238Å c: 125.664Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.147 0.212
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

17 review citations

The type III secretion system of Pseudomonas aeruginosa: infection by injection.
Hauser AR. (2009)
16 more

17 mentions without citation

Modulators of 14-3-3 Protein-Protein Interactions.
Stevers et al. (2018)
16 more