› 2o1o

X-ray diffraction
2.42Å resolution

Cryptosporidium parvum putative polyprenyl pyrophosphate synthase (cgd4_2550) in complex with risedronate.

Released:
DOI: 10.2210/pdb2o1o/pdb
PDB entry 2o1o coloured by chain, front view.
PDB entry 2o1o coloured by chain, side view.
PDB entry 2o1o coloured by chain, top view.
Source organism: Cryptosporidium parvum
Primary publication:
Targeting a uniquely nonspecific prenyl synthase with bisphosphonates to combat cryptosporidiosis.
Artz JD, Dunford JE, Arrowood MJ, Dong A, Chruszcz M, Kavanagh KL, Minor W, Russell RG, Ebetino FH, Oppermann U, Hui R
Chem Biol 15 1296-306 (2008)
PMID: 19101474

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176918 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative farnesyl pyrophosphate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 368 amino acids
Theoretical weight: 42.97 KDa
Source organism: Cryptosporidium parvum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5CR09 (Residues: 38-384; Coverage: 90%)
Gene name: cgd4_2550
Sequence domains: Polyprenyl synthetase
Structure domains: Farnesyl Diphosphate Synthase

Ligands and Environments

2 bound ligands:
Ligand MG 6 x MG
Ligand RIS 2 x RIS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P31
Unit cell:
a: 101.758Å b: 101.758Å c: 75.313Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.186 0.213
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Bisphosphonates: the first 40 years.
Russell RG. (2011)
8 more

2 mentions without citation

Benefits of structural genomics for drug discovery research.
Grabowski et al. (2009)
1 more