› 2o74

X-ray diffraction
1.8Å resolution

Structure of OHCU decarboxylase in complex with guanine

Released:
DOI: 10.2210/pdb2o74/pdb
PDB entry 2o74 coloured by chain, front view.
PDB entry 2o74 coloured by chain, side view.
PDB entry 2o74 coloured by chain, top view.
Source organism: Danio rerio
Primary publication:
The structure of 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase provides insights into the mechanism of uric acid degradation.
Cendron L, Berni R, Folli C, Ramazzina I, Percudani R, Zanotti G
J Biol Chem 282 18182-18189 (2007)
PMID: 17428786

Function and Biology Details

Reaction catalysed: 
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate = (S)-allantoin + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106657 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 174 amino acids
Theoretical weight: 19.77 KDa
Source organism: Danio rerio
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A1L259 (Residues: 1-174; Coverage: 100%)
Gene names: prhoxnb, urad, zgc:158663
Sequence domains: OHCU decarboxylase
Structure domains: Oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase

Ligands and Environments

1 bound ligand:
Ligand GUN 6 x GUN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P32
Unit cell:
a: 102.223Å b: 102.223Å c: 103.892Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.221 0.257
Expression system: Escherichia coli BL21

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Citations

1 review citation

A novel enzymatic approach in the production of food with low purine content using Arxula adeninivorans endogenous and recombinant purine degradative enzymes.
Jankowska et al. (2015)
24 other articles