› 2o7m

X-ray diffraction
2Å resolution

The C-terminal loop of the homing endonuclease I-CreI is essential for DNA binding and cleavage. Identification of a novel site for specificity engineering in the I-CreI scaffold

Released:
DOI: 10.2210/pdb2o7m/pdb
PDB entry 2o7m coloured by chain, front view.
PDB entry 2o7m coloured by chain, side view.
PDB entry 2o7m coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
The C-terminal loop of the homing endonuclease I-CreI is essential for site recognition, DNA binding and cleavage.
Prieto J, Redondo P, Padró D, Arnould S, Epinat JC, Pâques F, Blanco FJ, Montoya G
Nucleic Acids Res 35 3262-71 (2007)
PMID: 17452357

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138749 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA endonuclease I-CreI Chains: A, B
Molecule details ›
Chains: A, B
Length: 156 amino acids
Theoretical weight: 17.93 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P05725 (Residues: 1-156; Coverage: 96%)
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4, SLS BEAMLINE X06SA
Spacegroup: P43
Unit cell:
a: 69.088Å b: 69.088Å c: 93.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.184 0.235
Expression system: Escherichia coli BL21

Quick links

Citations

7 review citations

Meganucleases and other tools for targeted genome engineering: perspectives and challenges for gene therapy.
Silva et al. (2011)
6 more

4 mentions without citation

A protein-DNA docking benchmark.
van Dijk et al. (2008)
3 more