› 2oa9

X-ray diffraction
1.5Å resolution

Restriction endonuclease MvaI in the absence of DNA

Released:
DOI: 10.2210/pdb2oa9/pdb
PDB entry 2oa9 coloured by chain, front view.
PDB entry 2oa9 coloured by chain, side view.
PDB entry 2oa9 coloured by chain, top view.
Source organism: Kocuria varians
Primary publication:
Restriction endonuclease MvaI is a monomer that recognizes its target sequence asymmetrically.
Kaus-Drobek M, Czapinska H, SokoĊ‚owska M, Tamulaitis G, Szczepanowski RH, Urbanke C, Siksnys V, Bochtler M
Nucleic Acids Res 35 2035-46 (2007)
PMID: 17344322

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186013 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MvaI/BcnI restriction endonuclease domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 28.7 KDa
Source organism: Kocuria varians
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RNV5 (Residues: 1-246; Coverage: 100%)
Gene name: mvaIR
Sequence domains: MvaI/BcnI restriction endonuclease family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CL 2 x CL
Ligand CD 15 x CD
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P21
Unit cell:
a: 58.499Å b: 63.281Å c: 71.892Å
α: 90° β: 100.77° γ: 90°
R-values:
R R work R free
0.186 0.184 0.212
Expression system: Escherichia coli

Quick links

Citations

22 citation in other articles

Structural and evolutionary classification of Type II restriction enzymes based on theoretical and experimental analyses.
Orlowski et al. (2008)
21 more

5 mentions without citation

Type II restriction endonucleases--a historical perspective and more.
Pingoud et al. (2014)
4 more