› 2oar

X-ray diffraction
3.5Å resolution

Mechanosensitive Channel of Large Conductance (MscL)

Released:
DOI: 10.2210/pdb2oar/pdb
PDB entry 2oar coloured by chain, front view.
PDB entry 2oar coloured by chain, side view.
PDB entry 2oar coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Ra
Primary publication:
Structures of the Prokaryotic Mechanosensitive Channels MscL and MscS
Steinbacher S, Bass R, Strop P, Rees DC
Current Topics in Membranes 58 (2007)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo pentamer (preferred)
homo decamer
Assembly name:
PDBe Complex ID:
PDB-CPX-107728 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Large-conductance mechanosensitive channel Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 174 amino acids
Theoretical weight: 18.82 KDa
Source organism: Mycobacterium tuberculosis H37Ra
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A5U127 (Residues: 1-151; Coverage: 100%)
Gene names: MRA_0992, mscL
Sequence domains: Large-conductance mechanosensitive channel, MscL
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand AU 3 x AU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: I41
Unit cell:
a: 212.308Å b: 212.308Å c: 97.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.319 0.319 0.338
Expression system: Escherichia coli BL21

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Citations

87 mentions without citation

Normal mode analysis of biomolecular structures: functional mechanisms of membrane proteins.
Bahar et al. (2010)
86 more