› 2obl

X-ray diffraction
1.8Å resolution

Structural and biochemical analysis of a prototypical ATPase from the type III secretion system of pathogenic bacteria

Released:
DOI: 10.2210/pdb2obl/pdb
PDB entry 2obl coloured by chain, front view.
PDB entry 2obl coloured by chain, side view.
PDB entry 2obl coloured by chain, top view.
Source organism: Escherichia coli O127:H6 str. E2348/69
Primary publication:
Structural analysis of a prototypical ATPase from the type III secretion system.
Zarivach R, Vuckovic M, Deng W, Finlay BB, Strynadka NC
Nat Struct Mol Biol 14 131-7 (2007)
PMID: 17237797

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + cellular protein(Side 1) = ADP + phosphate + cellular protein(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110625 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
protein-secreting ATPase Chain: A
Molecule details ›
Chain: A
Length: 347 amino acids
Theoretical weight: 37.67 KDa
Source organism: Escherichia coli O127:H6 str. E2348/69
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: B7UMA6 (Residues: 103-446; Coverage: 77%)
Gene names: E2348C_3948, escN
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 1 x CA
Ligand ACT 1 x ACT
Ligand IMD 1 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21212
Unit cell:
a: 84.382Å b: 77.609Å c: 52.901Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.232
Expression system: Escherichia coli BL21

Quick links

Citations

25 review citations

Secretion systems in Gram-negative bacteria: structural and mechanistic insights.
Costa et al. (2015)
24 more

8 mentions without citation

Bacterial nanomachines: the flagellum and type III injectisome.
Erhardt et al. (2010)
7 more