› 2oih

X-ray diffraction
2.4Å resolution

Hepatitis Delta Virus gemonic ribozyme precursor with C75U mutation and bound to monovalent cation Tl+

Released:
DOI: 10.2210/pdb2oih/pdb
PDB entry 2oih coloured by chain, front view.
PDB entry 2oih coloured by chain, side view.
PDB entry 2oih coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural roles of monovalent cations in the HDV ribozyme.
Ke A, Ding F, Batchelor JD, Doudna JA
Structure 15 281-7 (2007)
PMID: 17355864

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140478 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
U1 small nuclear ribonucleoprotein A Chain: A
Molecule details ›
Chain: A
Length: 100 amino acids
Theoretical weight: 11.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09012 (Residues: 2-100; Coverage: 35%)
Gene name: SNRPA
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
HDV ribozyme Chain: B
Molecule details ›
Chain: B
Length: 75 nucleotides
Theoretical weight: 24.16 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand TL 15 x TL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: R32
Unit cell:
a: 107.962Å b: 107.962Å c: 189.119Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.266 0.266 0.288
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

8 review citations

Ribozymes, riboswitches and beyond: regulation of gene expression without proteins.
Serganov et al. (2007)
7 more

2 mentions without citation

Two distinct catalytic strategies in the hepatitis δ virus ribozyme cleavage reaction.
Golden BL. (2011)
1 more