› 2one

X-ray diffraction
2Å resolution

ASYMMETRIC YEAST ENOLASE DIMER COMPLEXED WITH RESOLVED 2'-PHOSPHOGLYCERATE AND PHOSPHOENOLPYRUVATE

Released:
DOI: 10.2210/pdb2one/pdb
PDB entry 2one coloured by chain, front view.
PDB entry 2one coloured by chain, side view.
PDB entry 2one coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Mechanism of enolase: the crystal structure of asymmetric dimer enolase-2-phospho-D-glycerate/enolase-phosphoenolpyruvate at 2.0 A resolution.
Zhang E, Brewer JM, Minor W, Carreira LA, Lebioda L
Biochemistry 36 12526-34 (1997)
PMID: 9376357

Function and Biology Details

Reaction catalysed: 
2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133690 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enolase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 436 amino acids
Theoretical weight: 46.73 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P00924 (Residues: 2-437; Coverage: 100%)
Gene names: ENO1, ENOA, G9160, HSP48, YGR254W
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 2 x MG
Ligand LI 1 x LI
Ligand 2PG 1 x 2PG
Ligand PEP 1 x PEP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 63.1Å b: 110Å c: 66.2Å
α: 90° β: 113° γ: 90°
R-values:
R R work R free
0.137 0.137 not available

Quick links

Citations

3 review citations

α-Enolase, a multifunctional protein: its role on pathophysiological situations.
Díaz-Ramos et al. (2012)
2 more

10 mentions without citation

Benzotriazole: An overview on its versatile biological behavior.
Briguglio et al. (2015)
9 more