› 2ore

X-ray diffraction
2.99Å resolution

Binary Structure of Escherichia coli DNA Adenine Methyltransferase and S-adenosylhomocysteine

Released:
DOI: 10.2210/pdb2ore/pdb
PDB entry 2ore coloured by chain, front view.
PDB entry 2ore coloured by chain, side view.
PDB entry 2ore coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Two alternative conformations of S-adenosyl-L-homocysteine bound to Escherichia coli DNA adenine methyltransferase and the implication of conformational changes in regulating the catalytic cycle.
Liebert K, Horton JR, Chahar S, Orwick M, Cheng X, Jeltsch A
J Biol Chem 282 22848-55 (2007)
PMID: 17545164

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142448 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA adenine methylase Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 298 amino acids
Theoretical weight: 34.31 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEE8 (Residues: 1-278; Coverage: 100%)
Gene names: JW3350, b3387, dam
Sequence domains: D12 class N6 adenine-specific DNA methyltransferase
Structure domains:

Ligands and Environments


Cofactor: Ligand SAH 3 x SAH
1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P3121
Unit cell:
a: 160.135Å b: 160.135Å c: 94.004Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.202 0.235
Expression system: Escherichia coli

Quick links

Citations

8 citation in other articles

Insights into the hyperthermostability and unusual region-specificity of archaeal Pyrococcus abyssi tRNA m1A57/58 methyltransferase.
Guelorget et al. (2010)
7 more

2 mentions without citation

Bacterial DNA methyltransferase: A key to the epigenetic world with lessons learned from proteobacteria.
Gao et al. (2023)
1 more