› 2oto

X-ray diffraction
3.04Å resolution

N-terminal fragment of Streptococcus pyogenes M1 protein

Released:
DOI: 10.2210/pdb2oto/pdb
PDB entry 2oto coloured by chain, front view.
PDB entry 2oto coloured by chain, side view.
PDB entry 2oto coloured by chain, top view.
Source organism: Streptococcus pyogenes serotype M1
Primary publication:
Coiled-coil irregularities and instabilities in group A Streptococcus M1 are required for virulence.
McNamara C, Zinkernagel AS, Macheboeuf P, Cunningham MW, Nizet V, Ghosh P
Science 319 1405-8 (2008)
PMID: 18323455

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-163128 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
M protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 155 amino acids
Theoretical weight: 18.18 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2, APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 119.374Å b: 83.405Å c: 93.467Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.337 0.337 0.338
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Streptococcus adherence and colonization.
Nobbs et al. (2009)
20 more

7 mentions without citation

Structural attributes for the recognition of weak and anomalous regions in coiled-coils of myosins and other motor proteins.
Sunitha et al. (2012)
6 more