› 2ou7

X-ray diffraction
2.4Å resolution

Structure of the Catalytic Domain of Human Polo-like Kinase 1

Released:
DOI: 10.2210/pdb2ou7/pdb
PDB entry 2ou7 coloured by chain, front view.
PDB entry 2ou7 coloured by chain, side view.
PDB entry 2ou7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the catalytic domain of human polo-like kinase 1.
Kothe M, Kohls D, Low S, Coli R, Cheng AC, Jacques SL, Johnson TL, Lewis C, Loh C, Nonomiya J, Sheils AL, Verdries KA, Wynn TA, Kuhn C, Ding YH
Biochemistry 46 5960-71 (2007)
PMID: 17461553

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-156752 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase PLK1 Chain: A
Molecule details ›
Chain: A
Length: 335 amino acids
Theoretical weight: 37.63 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P53350 (Residues: 13-345; Coverage: 55%)
Gene names: PLK, PLK1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand ACT 2 x ACT
Ligand MG 2 x MG
Ligand ANP 1 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P3221
Unit cell:
a: 65.958Å b: 65.958Å c: 154.045Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.203 0.235
Expression system: Spodoptera frugiperda

Quick links

Citations

23 review citations

Multifaceted polo-like kinases: drug targets and antitargets for cancer therapy.
Strebhardt K. (2010)
22 more

8 mentions without citation

Spatial exclusivity combined with positive and negative selection of phosphorylation motifs is the basis for context-dependent mitotic signaling.
Alexander et al. (2011)
7 more