› 2oug

X-ray diffraction
2.1Å resolution

Crystal structure of the RfaH transcription factor at 2.1A resolution

Released:
DOI: 10.2210/pdb2oug/pdb
PDB entry 2oug coloured by chain, front view.
PDB entry 2oug coloured by chain, side view.
PDB entry 2oug coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis for converting a general transcription factor into an operon-specific virulence regulator.
Belogurov GA, Vassylyeva MN, Svetlov V, Klyuyev S, Grishin NV, Vassylyev DG, Artsimovitch I
Mol Cell 26 117-29 (2007)
PMID: 17434131

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142754 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription antitermination protein RfaH Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 162 amino acids
Theoretical weight: 18.36 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AFW0 (Residues: 1-162; Coverage: 100%)
Gene names: JW3818, b3842, hlyT, rfaH, sfrB
Sequence domains: Transcription termination factor nusG
Structure domains: NusG, N-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P65
Unit cell:
a: 45.149Å b: 45.149Å c: 600.163Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.242 0.238 0.273
Expression system: Escherichia coli

Quick links

Citations

25 review citations

Evolution of multisubunit RNA polymerases in the three domains of life.
Werner et al. (2011)
24 more

27 mentions without citation

NusG-Spt5 proteins-Universal tools for transcription modification and communication.
Tomar et al. (2013)
26 more