› 2ovw

X-ray diffraction
2.3Å resolution

ENDOGLUCANASE I COMPLEXED WITH CELLOBIOSE

Released:
DOI: 10.2210/pdb2ovw/pdb
PDB entry 2ovw coloured by chain, front view.
PDB entry 2ovw coloured by chain, side view.
PDB entry 2ovw coloured by chain, top view.
Source organism: Fusarium oxysporum
Primary publication:
Structure of the endoglucanase I from Fusarium oxysporum: native, cellobiose, and 3,4-epoxybutyl beta-D-cellobioside-inhibited forms, at 2.3 A resolution.
Sulzenbacher G, Schülein M, Davies GJ
Biochemistry 36 5902-11 (1997)
PMID: 9153432

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155450 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Endoglucanase type C Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 411 amino acids
Theoretical weight: 44.69 KDa
Source organism: Fusarium oxysporum
UniProt:
  • Canonical: P46237 (Residues: 19-429; Coverage: 100%)
Sequence domains: Glycosyl hydrolase family 7
Structure domains: Glycoside hydrolase, family 7, domain

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
1 bound ligand:
Ligand NAG 8 x NAG
1 modified residue:
Ligand PCA 4 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-C
Spacegroup: P21
Unit cell:
a: 67.93Å b: 78.31Å c: 142.47Å
α: 90° β: 96.98° γ: 90°
R-values:
R R work R free
0.209 0.209 not available

Quick links

Citations

5 review citations

Structural and sequence-based classification of glycoside hydrolases.
Henrissat et al. (1997)
4 more

7 mentions without citation

Energetics of galactose- and glucose-aromatic amino acid interactions: implications for binding in galactose-specific proteins.
Sujatha et al. (2004)
6 more