› 2p1h

X-ray diffraction
1.59Å resolution

Rapid Folding and Unfolding of Apaf-1 CARD

Released:
DOI: 10.2210/pdb2p1h/pdb
PDB entry 2p1h coloured by chain, front view.
PDB entry 2p1h coloured by chain, side view.
PDB entry 2p1h coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Rapid folding and unfolding of Apaf-1 CARD.
Milam SL, Nicely NI, Feeney B, Mattos C, Clark AC
J Mol Biol 369 290-304 (2007)
PMID: 17408690

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127061 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Apoptotic protease-activating factor 1 Chain: A
Molecule details ›
Chain: A
Length: 94 amino acids
Theoretical weight: 10.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14727 (Residues: 1-92; Coverage: 7%)
Gene names: APAF1, KIAA0413
Sequence domains: Caspase recruitment domain
Structure domains: Death Domain, Fas

Ligands and Environments

1 bound ligand:
Ligand ZN 6 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 33.468Å b: 47.515Å c: 54.746Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.239 0.297
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

Practical approaches to protein folding and assembly: spectroscopic strategies in thermodynamics and kinetics.
Walters et al. (2009)
4 more

1 mention without citation

Investigation of Ionization Potential in Quantum Dots Using the Stratified Stochastic Enumeration of Molecular Orbitals Method.
Spanedda et al. (2022)