› 2p1m

X-ray diffraction
1.8Å resolution

TIR1-ASK1 complex structure

Released:
DOI: 10.2210/pdb2p1m/pdb
PDB entry 2p1m coloured by chain, front view.
PDB entry 2p1m coloured by chain, side view.
PDB entry 2p1m coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Mechanism of auxin perception by the TIR1 ubiquitin ligase.
Tan X, Calderon-Villalobos LI, Sharon M, Zheng C, Robinson CV, Estelle M, Zheng N
Nature 446 640-5 (2007)
PMID: 17410169

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-174445 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SKP1-like protein 1A Chain: A
Molecule details ›
Chain: A
Length: 160 amino acids
Theoretical weight: 17.88 KDa
Source organism: Arabidopsis thaliana
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q39255 (Residues: 1-160; Coverage: 100%)
Gene names: ASK1, At1g75950, SKP1, SKP1A, T4O12.17, UIP1
Sequence domains:
Structure domains: Potassium Channel Kv1.1; Chain A
Protein TRANSPORT INHIBITOR RESPONSE 1 Chain: B
Molecule details ›
Chain: B
Length: 594 amino acids
Theoretical weight: 66.88 KDa
Source organism: Arabidopsis thaliana
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q570C0 (Residues: 1-594; Coverage: 100%)
Gene names: At3g62980, FBL1, T20O10.80, TIR1, WEI1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand IHP 1 x IHP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2
Unit cell:
a: 102.51Å b: 80.45Å c: 125.031Å
α: 90° β: 105.11° γ: 90°
R-values:
R R work R free
0.187 0.185 0.233
Expression system: Spodoptera frugiperda

Quick links

Citations

267 review citations

RING domain E3 ubiquitin ligases.
Deshaies et al. (2009)
266 more

9 mentions without citation

Comparative Geometrical Analysis of Leucine-Rich Repeat Structures in the Nod-Like and Toll-Like Receptors in Vertebrate Innate Immunity.
Matsushima et al. (2015)
8 more