› 2p3x

X-ray diffraction
2.2Å resolution

Crystal structure of Grenache (Vitis vinifera) Polyphenol Oxidase

Released:
DOI: 10.2210/pdb2p3x/pdb
PDB entry 2p3x coloured by chain, front view.
PDB entry 2p3x coloured by chain, side view.
PDB entry 2p3x coloured by chain, top view.
Source organism: Vitis vinifera
Primary publication:
Cloning, sequencing, purification, and crystal structure of Grenache (Vitis vinifera) polyphenol oxidase.
Virador VM, Reyes Grajeda JP, Blanco-Labra A, Mendiola-Olaya E, Smith GM, Moreno A, Whitaker JR
J Agric Food Chem 58 1189-201 (2010)
PMID: 20039636

Function and Biology Details

Reaction catalysed: 
2 catechol + O(2) = 2 1,2-benzoquinone + 2 H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155023 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyphenol oxidase, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 339 amino acids
Theoretical weight: 38.47 KDa
Source organism: Vitis vinifera
UniProt:
  • Canonical: P43311 (Residues: 104-442; Coverage: 56%)
Sequence domains:
Structure domains: Di-copper center containing domain from catechol oxidase

Ligands and Environments

1 bound ligand:
Ligand C2O 1 x C2O
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: C2221
Unit cell:
a: 60.03Å b: 120.77Å c: 140.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.201 0.252

Quick links

Citations

7 review citations

Copper-O2 reactivity of tyrosinase models towards external monophenolic substrates: molecular mechanism and comparison with the enzyme.
Rolff et al. (2011)
6 more

21 mentions without citation

Copper active sites in biology.
Solomon et al. (2014)
20 more