› 2paf

X-ray diffraction
3.5Å resolution

Crystal Structure of the Lactose Repressor bound to anti-inducer ONPF in induced state

Released:
DOI: 10.2210/pdb2paf/pdb
PDB entry 2paf coloured by chain, front view.
PDB entry 2paf coloured by chain, side view.
PDB entry 2paf coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural analysis of lac repressor bound to allosteric effectors.
Daber R, Stayrook S, Rosenberg A, Lewis M
J Mol Biol 370 609-19 (2007)
PMID: 17543986

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136465 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lactose operon repressor Chains: A, B
Molecule details ›
Chains: A, B
Length: 269 amino acids
Theoretical weight: 28.76 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03023 (Residues: 62-330; Coverage: 75%)
Gene names: JW0336, b0345, lacI
Sequence domains: Periplasmic binding protein-like domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand NPF 2 x NPF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P41212
Unit cell:
a: 77.541Å b: 77.541Å c: 212.456Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.195 0.265
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Coordination chemistry of bacterial metal transport and sensing.
Ma et al. (2009)
4 more

7 mentions without citation

Structural analysis of lac repressor bound to allosteric effectors.
Daber et al. (2007)
6 more