› 2pbw

X-ray diffraction
2.5Å resolution

Crystal Structure of the Ligand-Binding Core of iGluR5 in Complex with the Partial agonist Domoic Acid at 2.5 A Resolution

Released:
DOI: 10.2210/pdb2pbw/pdb
PDB entry 2pbw coloured by chain, front view.
PDB entry 2pbw coloured by chain, side view.
PDB entry 2pbw coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Partial agonism and antagonism of the ionotropic glutamate receptor iGLuR5: structures of the ligand-binding core in complex with domoic acid and 2-amino-3-[5-tert-butyl-3-(phosphonomethoxy)-4-isoxazolyl]propionic acid.
Hald H, Naur P, Pickering DS, Sprogøe D, Madsen U, Timmermann DB, Ahring PK, Liljefors T, Schousboe A, Egebjerg J, Gajhede M, Kastrup JS
J Biol Chem 282 25726-36 (2007)
PMID: 17581823

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149794 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate receptor ionotropic, kainate 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 257 amino acids
Theoretical weight: 29.11 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P22756 (Residues: 445-559, 682-820; Coverage: 28%)
Gene names: Glur5, Grik1
Sequence domains:
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

1 bound ligand:
Ligand DOQ 2 x DOQ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P41212
Unit cell:
a: 70.863Å b: 70.863Å c: 233.168Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.209 0.248
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Glutamate receptor ion channels: structure, regulation, and function.
Traynelis et al. (2010)
8 more

5 mentions without citation

Ligand pose and orientational sampling in molecular docking.
Coleman et al. (2013)
4 more