› 2pda

X-ray diffraction
3Å resolution

CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN PYRUVATE-FERREDOXIN OXIDOREDUCTASE FROM DESULFOVIBRIO AFRICANUS AND PYRUVATE.

Released:
DOI: 10.2210/pdb2pda/pdb
PDB entry 2pda coloured by chain, front view.
PDB entry 2pda coloured by chain, side view.
PDB entry 2pda coloured by chain, top view.
Source organism: Desulfocurvibacter africanus
Primary publication:
Crystal structures of the key anaerobic enzyme pyruvate:ferredoxin oxidoreductase, free and in complex with pyruvate.
Chabrière E, Charon MH, Volbeda A, Pieulle L, Hatchikian EC, Fontecilla-Camps JC
Nat Struct Biol 6 182-90 (1999)
PMID: 10048931

Function and Biology Details

Reaction catalysed: 
Pyruvate + CoA + 2 oxidized ferredoxin = acetyl-CoA + CO(2) + 2 reduced ferredoxin + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161130 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments


Cofactor: Ligand TPP 2 x TPP
4 bound ligands:
Ligand MG 2 x MG
Ligand CA 2 x CA
Ligand SF4 6 x SF4
Ligand PYR 2 x PYR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 86Å b: 146.3Å c: 211.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 0.297

Quick links

Citations

22 review citations

Acetogenesis and the Wood-Ljungdahl pathway of CO(2) fixation.
Ragsdale et al. (2008)
21 more

3 mentions without citation

A tale of two ferredoxins: sequence similarity and structural differences.
Krishna et al. (2006)
2 more