› 2pe8

X-ray diffraction
2Å resolution

Crystal structure of the UHM domain of human SPF45 (free form)

Released:
DOI: 10.2210/pdb2pe8/pdb
PDB entry 2pe8 coloured by chain, front view.
PDB entry 2pe8 coloured by chain, side view.
PDB entry 2pe8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
U2AF-homology motif interactions are required for alternative splicing regulation by SPF45.
Corsini L, Bonnal S, Bonna S, Basquin J, Hothorn M, Scheffzek K, Valcárcel J, Sattler M
Nat Struct Mol Biol 14 620-9 (2007)
PMID: 17589525

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188542 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Splicing factor 45 Chain: A
Molecule details ›
Chain: A
Length: 105 amino acids
Theoretical weight: 11.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96I25 (Residues: 301-401; Coverage: 25%)
Gene names: RBM17, SPF45
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: F222
Unit cell:
a: 63.9Å b: 90.2Å c: 99.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.231 0.243
Expression system: Escherichia coli

Quick links

Citations

24 review citations

RNA recognition motifs: boring? Not quite.
Cléry et al. (2008)
23 more

4 mentions without citation

Thioredoxin as a fusion tag for carrier-driven crystallization.
Corsini et al. (2008)
3 more