› 2pee

X-ray diffraction
2.7Å resolution

Crystal Structure of a Thermophilic Serpin, Tengpin, in the Native State

Released:
DOI: 10.2210/pdb2pee/pdb
PDB entry 2pee coloured by chain, front view.
PDB entry 2pee coloured by chain, side view.
PDB entry 2pee coloured by chain, top view.
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis MB4
Primary publication:
The N terminus of the serpin, tengpin, functions to trap the metastable native state.
Zhang Q, Buckle AM, Law RH, Pearce MC, Cabrita LD, Lloyd GJ, Irving JA, Smith AI, Ruzyla K, Rossjohn J, Bottomley SP, Whisstock JC
EMBO Rep 8 658-63 (2007)
PMID: 17557112

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo hexamer
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-185939 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serpin domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 387 amino acids
Theoretical weight: 43.71 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis MB4
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8R9P5 (Residues: 36-422; Coverage: 92%)
Gene name: TTE1560
Sequence domains: Serpin (serine protease inhibitor)
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: I23
Unit cell:
a: 217.939Å b: 217.939Å c: 217.939Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.235
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Serpins flex their muscle: II. Structural insights into target peptidase recognition, polymerization, and transport functions.
Whisstock et al. (2010)
5 more

7 mentions without citation

Prokaryote-derived protein inhibitors of peptidases: A sketchy occurrence and mostly unknown function.
Kantyka et al. (2010)
6 more