› 2phk

X-ray diffraction
2.6Å resolution

THE CRYSTAL STRUCTURE OF A PHOSPHORYLASE KINASE PEPTIDE SUBSTRATE COMPLEX: KINASE SUBSTRATE RECOGNITION

Released:
DOI: 10.2210/pdb2phk/pdb
PDB entry 2phk coloured by chain, front view.
PDB entry 2phk coloured by chain, side view.
PDB entry 2phk coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
The crystal structure of a phosphorylase kinase peptide substrate complex: kinase substrate recognition.
Lowe ED, Noble ME, Skamnaki VT, Oikonomakos NG, Owen DJ, Johnson LN
EMBO J 16 6646-58 (1997)
PMID: 9362479

Function and Biology Details

Reactions catalysed: 
ATP + a protein = ADP + a phosphoprotein
2 ATP + phosphorylase b = 2 ADP + phosphorylase a
ATP + [tau protein] = ADP + [tau protein] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132601 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform Chain: A
Molecule details ›
Chain: A
Length: 277 amino acids
Theoretical weight: 31.98 KDa
Source organism: Oryctolagus cuniculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00518 (Residues: 15-291; Coverage: 72%)
Gene names: PHKG, PHKG1
Sequence domains: Protein kinase domain
Structure domains:
MC-PEPTIDE Chain: B
Molecule details ›
Chain: B
Length: 7 amino acids
Theoretical weight: 939 Da
Source organism: Oryctolagus cuniculus
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand MN 2 x MN
Ligand ATP 1 x ATP
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM02
Spacegroup: P3221
Unit cell:
a: 65.3Å b: 65.3Å c: 145.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.253 0.236 0.3
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

22 review citations

Mechanisms of specificity in protein phosphorylation.
Ubersax et al. (2007)
21 more

26 mentions without citation

Substrate and docking interactions in serine/threonine protein kinases.
Goldsmith et al. (2007)
25 more