› 2pis

X-ray diffraction
2.8Å resolution

Efforts toward Expansion of the Genetic Alphabet: Structure and Replication of Unnatural Base Pairs

Released:
DOI: 10.2210/pdb2pis/pdb
PDB entry 2pis coloured by chain, front view.
PDB entry 2pis coloured by chain, side view.
PDB entry 2pis coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Efforts toward expansion of the genetic alphabet: structure and replication of unnatural base pairs.
Matsuda S, Fillo JD, Henry AA, Rai P, Wilkens SJ, Dwyer TJ, Geierstanger BH, Wemmer DE, Schultz PG, Spraggon G, Romesberg FE
J Am Chem Soc 129 10466-73 (2007)
PMID: 17685517

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115768 (preferred)
Entry contents:
1 distinct DNA molecule
Macromolecule:
DNA (5'-D(*CP*GP*(CBR)P*GP*AP*AP*(FFD)P*TP*TP*CP*GP*CP*G)-3') Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 13 nucleotides
Theoretical weight: 4.02 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 6 x MG
1 modified residue:
Ligand CBR 12 x CBR

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: I4122
Unit cell:
a: 146.025Å b: 146.025Å c: 93.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.224 0.303
Expression system: Not provided

Quick links

Citations

10 review citations

A gripping tale of ribosomal frameshifting: extragenic suppressors of frameshift mutations spotlight P-site realignment.
Atkins et al. (2009)
9 more

4 mentions without citation

New insights into Hoogsteen base pairs in DNA duplexes from a structure-based survey.
Zhou et al. (2015)
3 more