› 2pk2

X-ray diffraction
2.67Å resolution

Cyclin box structure of the P-TEFb subunit Cyclin T1 derived from a fusion complex with EIAV Tat

Released:
DOI: 10.2210/pdb2pk2/pdb
PDB entry 2pk2 coloured by chain, front view.
PDB entry 2pk2 coloured by chain, side view.
PDB entry 2pk2 coloured by chain, top view.
Source organisms:
Primary publication:
Cyclin box structure of the P-TEFb subunit cyclin T1 derived from a fusion complex with EIAV tat.
Anand K, Schulte A, Fujinaga K, Scheffzek K, Geyer M
J Mol Biol 370 826-36 (2007)
PMID: 17540406

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130017 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclin-T1; Protein Tat Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 358 amino acids
Theoretical weight: 40.34 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O60563 (Residues: 1-281; Coverage: 39%)
  • Canonical: P32544 (Residues: 19-75; Coverage: 76%)
Gene names: CCNT1, tat
Sequence domains:
Structure domains: Cyclin-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: R3
Unit cell:
a: 203.83Å b: 203.83Å c: 124.79Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.274 0.274 0.306
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Non-coding RNAs regulating the transcriptional machinery.
Barrandon et al. (2008)
3 more

6 mentions without citation

Tetartohedral twinning could happen to you too.
Roversi et al. (2012)
5 more