› 2pkt

X-ray diffraction
1.5Å resolution

Crystal structure of the human CLP-36 (PDLIM1) bound to the C-terminal peptide of human alpha-actinin-1

Released:
DOI: 10.2210/pdb2pkt/pdb
PDB entry 2pkt coloured by chain, front view.
PDB entry 2pkt coloured by chain, side view.
PDB entry 2pkt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Unusual binding interactions in PDZ domain crystal structures help explain binding mechanisms.
Elkins JM, Gileadi C, Shrestha L, Phillips C, Wang J, Muniz JR, Doyle DA
Protein Sci 19 731-41 (2010)
PMID: 20120020

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126122 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PDZ and LIM domain protein 1 Chain: A
Molecule details ›
Chain: A
Length: 91 amino acids
Theoretical weight: 9.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O00151 (Residues: 1-87; Coverage: 26%)
Gene names: CLIM1, CLP36, PDLIM1
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain

Ligands and Environments

5 bound ligands:
Ligand CA 2 x CA
Ligand CL 1 x CL
Ligand ACT 1 x ACT
Ligand PG4 1 x PG4
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P6522
Unit cell:
a: 38.74Å b: 38.74Å c: 246.46Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.213 0.212 0.231
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

PDZ Domains as Drug Targets.
Christensen et al. (2019)
3 more

10 mentions without citation

PDLIM1: Structure, function and implication in cancer.
Zhou et al. (2021)
9 more