› 2plq

X-ray diffraction
1.9Å resolution

Crystal structure of the amidase from geobacillus pallidus RAPc8

Released:
DOI: 10.2210/pdb2plq/pdb
PDB entry 2plq coloured by chain, front view.
PDB entry 2plq coloured by chain, side view.
PDB entry 2plq coloured by chain, top view.
Source organism: Aeribacillus pallidus
Primary publication:
The quaternary structure of the amidase from Geobacillus pallidus RAPc8 is revealed by its crystal packing.
Agarkar VB, Kimani SW, Cowan DA, Sayed MF, Sewell BT
Acta Crystallogr Sect F Struct Biol Cryst Commun 62 1174-8 (2006)
PMID: 17142891

Function and Biology Details

Reaction catalysed: 
A monocarboxylic acid amide + H(2)O = a monocarboxylate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192207 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aliphatic amidase Chain: A
Molecule details ›
Chain: A
Length: 348 amino acids
Theoretical weight: 38.64 KDa
Source organism: Aeribacillus pallidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9L543 (Residues: 1-348; Coverage: 100%)
Gene names: ami, amiE
Sequence domains: Carbon-nitrogen hydrolase
Structure domains: Carbon-nitrogen hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P4232
Unit cell:
a: 130.398Å b: 130.398Å c: 130.398Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.146 0.177
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Carbamoylases: characteristics and applications in biotechnological processes.
Martínez-Rodríguez et al. (2010)
2 other articles

5 mentions without citation

Symmetry-restrained flexible fitting for symmetric EM maps.
Chan et al. (2011)
4 more