› 2pth

X-ray diffraction
1.2Å resolution

PEPTIDYL-TRNA HYDROLASE FROM ESCHERICHIA COLI

Released:
DOI: 10.2210/pdb2pth/pdb
PDB entry 2pth coloured by chain, front view.
PDB entry 2pth coloured by chain, side view.
PDB entry 2pth coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystal structure at 1.2 A resolution and active site mapping of Escherichia coli peptidyl-tRNA hydrolase.
Schmitt E, Mechulam Y, Fromant M, Plateau P, Blanquet S
EMBO J 16 4760-9 (1997)
PMID: 9303320

Function and Biology Details

Reaction catalysed: 
N-substituted aminoacyl-tRNA + H(2)O = N-substituted amino acid + tRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141581 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidyl-tRNA hydrolase Chain: A
Molecule details ›
Chain: A
Length: 193 amino acids
Theoretical weight: 20.98 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0A7D1 (Residues: 2-194; Coverage: 100%)
Gene names: JW1195, b1204, pth
Sequence domains: Peptidyl-tRNA hydrolase
Structure domains: Peptidyl-tRNA hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE DW32
Spacegroup: P212121
Unit cell:
a: 47.24Å b: 63.59Å c: 62.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.215
Expression system: Escherichia coli K-12

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Citations

6 review citations

Initiation of mRNA translation in bacteria: structural and dynamic aspects.
Gualerzi et al. (2015)
5 more

37 mentions without citation

A graph-theory algorithm for rapid protein side-chain prediction.
Canutescu et al. (2003)
36 more