› 2q0j

X-ray diffraction
2.1Å resolution

Structure of Pseudomonas Quinolone Signal Response Protein PqsE

Released:
DOI: 10.2210/pdb2q0j/pdb
PDB entry 2q0j coloured by chain, front view.
PDB entry 2q0j coloured by chain, side view.
PDB entry 2q0j coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structure elucidation and preliminary assessment of hydrolase activity of PqsE, the Pseudomonas quinolone signal (PQS) response protein.
Yu S, Jensen V, Seeliger J, Feldmann I, Weber S, Schleicher E, Häussler S, Blankenfeldt W
Biochemistry 48 10298-307 (2009)
PMID: 19788310

Function and Biology Details

Reaction catalysed: 
2-aminobenzoylacetyl-CoA + H(2)O = 2-aminobenzoylacetate + CoA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149002 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-aminobenzoylacetyl-CoA thioesterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 321 amino acids
Theoretical weight: 36.52 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P20581 (Residues: 1-301; Coverage: 100%)
Gene names: PA1000, pqsE
Sequence domains: Metallo-beta-lactamase superfamily
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

2 bound ligands:
Ligand FE 4 x FE
Ligand BEZ 2 x BEZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 44.76Å b: 66.09Å c: 110.26Å
α: 90° β: 97.19° γ: 90°
R-values:
R R work R free
0.154 0.151 0.211
Expression system: Escherichia coli

Quick links

Citations

7 review citations

The multiple signaling systems regulating virulence in Pseudomonas aeruginosa.
Jimenez et al. (2012)
6 more

3 mentions without citation

Systematic mutational analysis of the putative hydrolase PqsE: toward a deeper molecular understanding of virulence acquisition in Pseudomonas aeruginosa.
Folch et al. (2013)
2 more