› 2q5h

X-ray diffraction
3Å resolution

Crystal structure of apo-wildtype Glycyl-tRNA synthetase

Released:
DOI: 10.2210/pdb2q5h/pdb
PDB entry 2q5h coloured by chain, front view.
PDB entry 2q5h coloured by chain, side view.
PDB entry 2q5h coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human wildtype and S581L-mutant glycyl-tRNA synthetase, an enzyme underlying distal spinal muscular atrophy.
Cader MZ, Ren J, James PA, Bird LE, Talbot K, Stammers DK
FEBS Lett 581 2959-64 (2007)
PMID: 17544401

Function and Biology Details

Reaction catalysed: 
ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154355 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 691 amino acids
Theoretical weight: 78.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P41250 (Residues: 55-739; Coverage: 93%)
Gene names: GARS, GARS1
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P43212
Unit cell:
a: 92.53Å b: 92.53Å c: 246.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Aminoacyl tRNA synthetases and their connections to disease.
Park et al. (2008)
9 more

1 mention without citation

Identification of Putative Drug Targets in Highly Resistant Gram-Negative Bacteria; and Drug Discovery Against Glycyl-tRNA Synthetase as a New Target.
Fereshteh et al. (2023)