› 2q5t

X-ray diffraction
2.1Å resolution

Full-length Cholix toxin from Vibrio Cholerae

Released:
DOI: 10.2210/pdb2q5t/pdb
PDB entry 2q5t coloured by chain, front view.
PDB entry 2q5t coloured by chain, side view.
PDB entry 2q5t coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
Cholix toxin, a novel ADP-ribosylating factor from Vibrio cholerae.
Jørgensen R, Purdy AE, Fieldhouse RJ, Kimber MS, Bartlett DH, Merrill AR
J Biol Chem 283 10671-8 (2008)
PMID: 18276581

Function and Biology Details

Reaction catalysed: 
NAD(+) + diphthamide-[translation elongation factor 2] = nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177028 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cholix toxin Chain: A
Molecule details ›
Chain: A
Length: 642 amino acids
Theoretical weight: 71.87 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5EK40 (Residues: 33-666; Coverage: 100%)
Gene names: chxA, toxA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand EDO 9 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR571
Spacegroup: P21
Unit cell:
a: 50.936Å b: 88.338Å c: 79.934Å
α: 90° β: 95.32° γ: 90°
R-values:
R R work R free
0.194 0.191 0.239
Expression system: Escherichia coli

Quick links

Citations

28 review citations

Toward a unified nomenclature for mammalian ADP-ribosyltransferases.
Hottiger et al. (2010)
27 more

7 mentions without citation

Novel cholix toxin variants, ADP-ribosylating toxins in Vibrio cholerae non-O1/non-O139 strains, and their pathogenicity.
Awasthi et al. (2013)
6 more