› 2q6i

X-ray diffraction
2.6Å resolution

salL with ClDA and LMet

Released:
DOI: 10.2210/pdb2q6i/pdb
PDB entry 2q6i coloured by chain, front view.
PDB entry 2q6i coloured by chain, side view.
PDB entry 2q6i coloured by chain, top view.
Source organism: Salinispora tropica CNB-440
Primary publication:
Discovery and characterization of a marine bacterial SAM-dependent chlorinase.
Eustáquio AS, Pojer F, Noel JP, Moore BS
Nat Chem Biol 4 69-74 (2008)
PMID: 18059261

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + chloride = 5-deoxy-5-chloroadenosine + L-methionine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-107475 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosyl-chloride synthase Chain: A
Molecule details ›
Chain: A
Length: 283 amino acids
Theoretical weight: 30.18 KDa
Source organism: Salinispora tropica CNB-440
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A4X3Q0 (Residues: 1-283; Coverage: 100%)
Gene names: Strop_1026, salL
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand 5CD 1 x 5CD
Ligand MET 1 x MET
Ligand PEG 3 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P6322
Unit cell:
a: 111.03Å b: 111.03Å c: 93.31Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.206 0.262
Expression system: Escherichia coli BL21

Quick links

Citations

41 review citations

Genomic basis for natural product biosynthetic diversity in the actinomycetes.
Nett et al. (2009)
40 more

8 mentions without citation

Structural perspective on enzymatic halogenation.
Blasiak et al. (2009)
7 more