› 2q9q

X-ray diffraction
2.36Å resolution

The crystal structure of full length human GINS complex

Released:
DOI: 10.2210/pdb2q9q/pdb
PDB entry 2q9q coloured by chain, front view.
PDB entry 2q9q coloured by chain, side view.
PDB entry 2q9q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the GINS complex and functional insights into its role in DNA replication.
Chang YP, Wang G, Bermudez V, Hurwitz J, Chen XS
Proc Natl Acad Sci U S A 104 12685-90 (2007)
PMID: 17652513

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172086 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
DNA replication complex GINS protein PSF2 Chains: A, E
Molecule details ›
Chains: A, E
Length: 191 amino acids
Theoretical weight: 21.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y248 (Residues: 1-185; Coverage: 100%)
Gene names: CGI-122, DC5, GINS2, HSPC037, PSF2
Sequence domains: GINS complex protein
Structure domains:
DNA replication complex GINS protein SLD5 Chains: B, F
Molecule details ›
Chains: B, F
Length: 223 amino acids
Theoretical weight: 26.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BRT9 (Residues: 1-223; Coverage: 100%)
Gene names: GINS4, SLD5
Sequence domains:
Structure domains:
DNA replication complex GINS protein PSF1 Chains: C, G
Molecule details ›
Chains: C, G
Length: 196 amino acids
Theoretical weight: 23.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14691 (Residues: 1-196; Coverage: 100%)
Gene names: GINS1, KIAA0186, PSF1
Sequence domains: GINS complex protein
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1
DNA replication complex GINS protein PSF3 Chains: D, H
Molecule details ›
Chains: D, H
Length: 220 amino acids
Theoretical weight: 24.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BRX5 (Residues: 2-216; Coverage: 100%)
Gene names: GINS3, PSF3
Sequence domains: GINS complex protein
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 58.269Å b: 89.096Å c: 103.8Å
α: 105.02° β: 103.58° γ: 95.07°
R-values:
R R work R free
0.21 0.21 0.248
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Eukaryotic chromosome DNA replication: where, when, and how?
Masai et al. (2010)
13 more

11 mentions without citation

Congenital Diseases of DNA Replication: Clinical Phenotypes and Molecular Mechanisms.
Schmit et al. (2021)
10 more