› 2qc9

X-ray diffraction
2.35Å resolution

Mouse Notch 1 Ankyrin Repeat Intracellular Domain

Released:
DOI: 10.2210/pdb2qc9/pdb
PDB entry 2qc9 coloured by chain, front view.
PDB entry 2qc9 coloured by chain, side view.
PDB entry 2qc9 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Asparaginyl hydroxylation of the Notch ankyrin repeat domain by factor inhibiting hypoxia-inducible factor.
Coleman ML, McDonough MA, Hewitson KS, Coles C, Mecinovic J, Edelmann M, Cook KM, Cockman ME, Lancaster DE, Kessler BM, Oldham NJ, Ratcliffe PJ, Schofield CJ
J Biol Chem 282 24027-38 (2007)
PMID: 17573339

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169617 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Notch 1 intracellular domain Chains: A, B
Molecule details ›
Chains: A, B
Length: 210 amino acids
Theoretical weight: 23.13 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q01705 (Residues: 1899-2106; Coverage: 8%)
Gene names: Motch, Notch1
Sequence domains:
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand AHB 2 x AHB

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P65
Unit cell:
a: 98.203Å b: 98.203Å c: 108.703Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.173 0.228
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

60 review citations

Oxygen sensing by metazoans: the central role of the HIF hydroxylase pathway.
Kaelin et al. (2008)
59 more

3 mentions without citation

The molecular logic of Notch signaling--a structural and biochemical perspective.
Gordon et al. (2008)
2 more