› 2qcs

X-ray diffraction
2.2Å resolution

A complex structure between the Catalytic and Regulatory subunit of Protein Kinase A that represents the inhibited state

Released:
DOI: 10.2210/pdb2qcs/pdb
PDB entry 2qcs coloured by chain, front view.
PDB entry 2qcs coloured by chain, side view.
PDB entry 2qcs coloured by chain, top view.
Source organisms:
Primary publication:
PKA-I holoenzyme structure reveals a mechanism for cAMP-dependent activation.
Kim C, Cheng CY, Saldanha SA, Taylor SS
Cell 130 1032-43 (2007)
PMID: 17889648

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132579 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
cAMP-dependent protein kinase catalytic subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 40.74 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P05132 (Residues: 2-351; Coverage: 100%)
Gene names: Pkaca, Prkaca
Sequence domains: Protein kinase domain
Structure domains:
cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processed Chain: B
Molecule details ›
Chain: B
Length: 291 amino acids
Theoretical weight: 32.72 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00514 (Residues: 91-380; Coverage: 76%)
Gene name: PRKAR1A
Sequence domains: Cyclic nucleotide-binding domain
Structure domains: Jelly Rolls

Ligands and Environments

6 bound ligands:
Ligand MN 2 x MN
Ligand SO4 6 x SO4
Ligand ACT 1 x ACT
Ligand ANP 1 x ANP
Ligand TAM 1 x TAM
Ligand GOL 3 x GOL
2 modified residues:
Ligand SEP 2 x SEP
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3221
Unit cell:
a: 125.809Å b: 125.809Å c: 140.941Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.192 0.225
Expression system: Escherichia coli BL21

Quick links

Citations

41 review citations

Protein kinases: evolution of dynamic regulatory proteins.
Taylor et al. (2011)
40 more

44 mentions without citation

Signaling through cAMP and cAMP-dependent protein kinase: diverse strategies for drug design.
Taylor et al. (2008)
43 more