› 2qic

X-ray diffraction
2.1Å resolution

Crystal Structure of the ING1 PHD Finger in complex with a Histone H3K4ME3 peptide

Released:
DOI: 10.2210/pdb2qic/pdb
PDB entry 2qic coloured by chain, front view.
PDB entry 2qic coloured by chain, side view.
PDB entry 2qic coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Histone H3K4me3 binding is required for the DNA repair and apoptotic activities of ING1 tumor suppressor.
Peña PV, Hom RA, Hung T, Lin H, Kuo AJ, Wong RP, Subach OM, Champagne KS, Zhao R, Verkhusha VV, Li G, Gozani O, Kutateladze TG
J Mol Biol 380 303-12 (2008)
PMID: 18533182

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-163196 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Inhibitor of growth protein 1 Chain: A
Molecule details ›
Chain: A
Length: 62 amino acids
Theoretical weight: 7.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UK53 (Residues: 345-404; Coverage: 14%)
Gene name: ING1
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)
H3K4ME3 PEPTIDE Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.35 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
1 modified residue:
Ligand M3L 1 x M3L

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P3221
Unit cell:
a: 49.06Å b: 49.06Å c: 52.62Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.24 0.24 0.264
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

32 review citations

Covalent histone modifications--miswritten, misinterpreted and mis-erased in human cancers.
Chi et al. (2010)
31 more

8 mentions without citation

PHD fingers: epigenetic effectors and potential drug targets.
Musselman et al. (2009)
7 more