› 2qlx

X-ray diffraction
2Å resolution

Crystal structure of rhamnose mutarotase RhaU of Rhizobium leguminosarum in complex with L-Rhamnose

Released:
DOI: 10.2210/pdb2qlx/pdb
PDB entry 2qlx coloured by chain, front view.
PDB entry 2qlx coloured by chain, side view.
PDB entry 2qlx coloured by chain, top view.
Source organism: Rhizobium leguminosarum bv. trifolii
Primary publication:
RhaU of Rhizobium leguminosarum is a rhamnose mutarotase.
Richardson JS, Carpena X, Switala J, Perez-Luque R, Donald LJ, Loewen PC, Oresnik IJ
J Bacteriol 190 2903-10 (2008)
PMID: 18156270

Function and Biology Details

Reaction catalysed: 
Alpha-L-rhamnopyranose = beta-L-rhamnopyranose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181372 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-rhamnose mutarotase Chains: A, B
Molecule details ›
Chains: A, B
Length: 108 amino acids
Theoretical weight: 12.86 KDa
Source organism: Rhizobium leguminosarum bv. trifolii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7BSH1 (Residues: 1-106; Coverage: 100%)
Gene names: rhaM, rhaU
Sequence domains: L-rhamnose mutarotase
Structure domains: Alpha-Beta Plaits

Ligands and Environments

3 bound ligands:
Ligand RM4 2 x RM4
Ligand MG 2 x MG
Ligand FMT 15 x FMT
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P3212
Unit cell:
a: 68.652Å b: 68.652Å c: 100.719Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.138 0.135 0.188
Expression system: Escherichia coli

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Citations

1 review citation

Physiology, genetics, and biochemistry of carbon metabolism in the alphaproteobacterium Sinorhizobium meliloti.
Geddes et al. (2014)
11 other articles

2 mentions without citation

DG-AMMOS: a new tool to generate 3d conformation of small molecules using distance geometry and automated molecular mechanics optimization for in silico screening.
Lagorce et al. (2009)
1 more