› 2qm4

X-ray diffraction
2.3Å resolution

Crystal structure of human XLF/Cernunnos, a non-homologous end-joining factor

Released:
DOI: 10.2210/pdb2qm4/pdb
PDB entry 2qm4 coloured by chain, front view.
PDB entry 2qm4 coloured by chain, side view.
PDB entry 2qm4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human XLF/Cernunnos reveals unexpected differences from XRCC4 with implications for NHEJ.
Li Y, Chirgadze DY, Bolanos-Garcia VM, Sibanda BL, Davies OR, Ahnesorg P, Jackson SP, Blundell TL
EMBO J 27 290-300 (2008)
PMID: 18046455

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190703 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Non-homologous end-joining factor 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 235 amino acids
Theoretical weight: 27.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H9Q4 (Residues: 1-233; Coverage: 78%)
Gene names: NHEJ1, XLF
Sequence domains: XLF-Cernunnos, XRcc4-like factor, NHEJ component
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 36 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 63.736Å b: 92.913Å c: 103.693Å
α: 90° β: 106.22° γ: 90°
R-values:
R R work R free
0.185 0.182 0.239
Expression system: Escherichia coli

Quick links

Citations

27 review citations

The mechanism of double-strand DNA break repair by the nonhomologous DNA end-joining pathway.
Lieber MR. (2010)
26 more

12 mentions without citation

Repair of double-strand breaks by end joining.
Chiruvella et al. (2013)
11 more