› 2qsw

X-ray diffraction
1.5Å resolution

Crystal structure of C-terminal domain of ABC transporter / ATP-binding protein from Enterococcus faecalis

Released:
DOI: 10.2210/pdb2qsw/pdb
PDB entry 2qsw coloured by chain, front view.
PDB entry 2qsw coloured by chain, side view.
PDB entry 2qsw coloured by chain, top view.
Source organism: Enterococcus faecalis V583
Entry authors: Osipiuk J, Bigelow L, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + D-methionine-[methionine-binding protein](Side 1) = ADP + phosphate + D-methionine(Side 2) + [methionine-binding protein](Side 1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182767 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionine import ATP-binding protein MetN 2 Chain: A
Molecule details ›
Chain: A
Length: 100 amino acids
Theoretical weight: 11.28 KDa
Source organism: Enterococcus faecalis V583
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q831K6 (Residues: 249-345; Coverage: 28%)
Gene names: EF_2498, metN2
Sequence domains: NIL domain
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand ZN 3 x ZN
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 1 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P4322
Unit cell:
a: 38.896Å b: 38.896Å c: 111.938Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.149 0.147 0.184
Expression system: Escherichia coli BL21(DE3)

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