› 2r0o

X-ray diffraction
2.2Å resolution

Crystal structure of the actin-binding domain of human alpha-actinin-4 mutant(K255E)

Released:
DOI: 10.2210/pdb2r0o/pdb
PDB entry 2r0o coloured by chain, front view.
PDB entry 2r0o coloured by chain, side view.
PDB entry 2r0o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the actin-binding domain of alpha-actinin-4 Lys255Glu mutant implicated in focal segmental glomerulosclerosis.
Lee SH, Weins A, Hayes DB, Pollak MR, Dominguez R
J Mol Biol 376 317-24 (2008)
PMID: 18164029

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129066 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-actinin-4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 237 amino acids
Theoretical weight: 27.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O43707 (Residues: 47-271; Coverage: 25%)
Gene name: ACTN4
Sequence domains: Calponin homology (CH) domain
Structure domains: Calponin-like domain

Ligands and Environments

1 bound ligand:
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P212121
Unit cell:
a: 61.697Å b: 61.533Å c: 174.936Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.171 0.224
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Regulation of actin cytoskeleton dynamics in cells.
Lee et al. (2010)
2 more

7 mentions without citation

Crystal structure of the actin-binding domain of alpha-actinin-4 Lys255Glu mutant implicated in focal segmental glomerulosclerosis.
Lee et al. (2008)
6 more