› 2r15

X-ray diffraction
2.24Å resolution

Crystal structure of the myomesin domains 12 and 13

Released:
DOI: 10.2210/pdb2r15/pdb
PDB entry 2r15 coloured by chain, front view.
PDB entry 2r15 coloured by chain, side view.
PDB entry 2r15 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis of the C-terminal tail-to-tail assembly of the sarcomeric filament protein myomesin.
Pinotsis N, Lange S, Perriard JC, Svergun DI, Wilmanns M
EMBO J 27 253-64 (2008)
PMID: 18059477

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156476 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myomesin-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 212 amino acids
Theoretical weight: 23.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52179 (Residues: 1459-1667; Coverage: 12%)
Gene name: MYOM1
Sequence domains: Immunoglobulin I-set domain
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand ACT 1 x ACT
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: C2221
Unit cell:
a: 61.504Å b: 87.87Å c: 203.081Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

The sarcomeric cytoskeleton: from molecules to motion.
Gautel et al. (2016)
6 more

8 mentions without citation

The sarcomeric M-region: a molecular command center for diverse cellular processes.
Hu et al. (2015)
7 more