› 2r85

X-ray diffraction
1.7Å resolution

Crystal structure of PurP from Pyrococcus furiosus complexed with AMP

Released:
DOI: 10.2210/pdb2r85/pdb
PDB entry 2r85 coloured by chain, front view.
PDB entry 2r85 coloured by chain, side view.
PDB entry 2r85 coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Crystal structure and function of 5-formaminoimidazole-4-carboxamide ribonucleotide synthetase from Methanocaldococcus jannaschii.
Zhang Y, White RH, Ealick SE
Biochemistry 47 205-17 (2008)
PMID: 18069798

Function and Biology Details

Reaction catalysed: 
ATP + formate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = ADP + phosphate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186382 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase Chains: A, B
Molecule details ›
Chains: A, B
Length: 334 amino acids
Theoretical weight: 38.35 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8U0R7 (Residues: 1-334; Coverage: 100%)
Gene names: PF1517, purP
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand NA 2 x NA
Ligand CL 2 x CL
Ligand AMP 4 x AMP
Ligand MPD 4 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: R32
Unit cell:
a: 123.205Å b: 123.205Å c: 376.298Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.167 0.165 0.186
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Divergence and convergence in enzyme evolution.
Galperin et al. (2012)
2 more

2 mentions without citation

Crystal structure and function of 5-formaminoimidazole-4-carboxamide ribonucleotide synthetase from Methanocaldococcus jannaschii.
Zhang et al. (2008)
1 more