› 2r9a

X-ray diffraction
2.5Å resolution

Crystal structure of human XLF

Released:
DOI: 10.2210/pdb2r9a/pdb
PDB entry 2r9a coloured by chain, front view.
PDB entry 2r9a coloured by chain, side view.
PDB entry 2r9a coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human XLF: a twist in nonhomologous DNA end-joining.
Andres SN, Modesti M, Tsai CJ, Chu G, Junop MS
Mol Cell 28 1093-101 (2007)
PMID: 18158905

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190703 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Non-homologous end-joining factor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 230 amino acids
Theoretical weight: 26.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H9Q4 (Residues: 1-224; Coverage: 75%)
Gene names: NHEJ1, XLF
Sequence domains: XLF-Cernunnos, XRcc4-like factor, NHEJ component
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P212121
Unit cell:
a: 63.46Å b: 86.95Å c: 91.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.295 0.251 0.287
Expression system: Escherichia coli

Quick links

Citations

28 review citations

The mechanism of double-strand DNA break repair by the nonhomologous DNA end-joining pathway.
Lieber MR. (2010)
27 more

14 mentions without citation

Nonhomologous end joining: a good solution for bad ends.
Waters et al. (2014)
13 more