› 2rap

X-ray diffraction
2.6Å resolution

THE SMALL G PROTEIN RAP2A IN COMPLEX WITH GTP

Released:
DOI: 10.2210/pdb2rap/pdb
PDB entry 2rap coloured by chain, front view.
PDB entry 2rap coloured by chain, side view.
PDB entry 2rap coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of the small G protein Rap2A in complex with its substrate GTP, with GDP and with GTPgammaS.
Cherfils J, Ménétrey J, Le Bras G, Janoueix-Lerosey I, de Gunzburg J, Garel JR, Auzat I
EMBO J 16 5582-91 (1997)
PMID: 9312017

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145172 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein Rap-2a Chain: A
Molecule details ›
Chain: A
Length: 167 amino acids
Theoretical weight: 18.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P10114 (Residues: 1-167; Coverage: 91%)
Gene name: RAP2A
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GTP 1 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE DW21B
Spacegroup: P61
Unit cell:
a: 64.5Å b: 64.5Å c: 84.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.206 0.281
Expression system: Escherichia coli K-12

Quick links

Citations

8 review citations

Regulation of small GTPases by GEFs, GAPs, and GDIs.
Cherfils et al. (2013)
7 more

5 mentions without citation

Ran's C-terminal, basic patch, and nucleotide exchange mechanisms in light of a canonical structure for Rab, Rho, Ras, and Ran GTPases.
Neuwald et al. (2003)
4 more