› 2ruh

Solution NMR

Chemical Shift Assignments for MIP and MDM2 in bound state

Released:
DOI: 10.2210/pdb2ruh/pdb
PDB entry 2ruh coloured by chain, ensemble of 20 models, front view.
PDB entry 2ruh coloured by chain, ensemble of 20 models, side view.
PDB entry 2ruh coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for inhibition of the MDM2:p53 interaction by an optimized MDM2-binding peptide selected with mRNA display.
Nagata T, Shirakawa K, Kobayashi N, Shiheido H, Tabata N, Sakuma-Yonemura Y, Horisawa K, Katahira M, Doi N, Yanagawa H
PLoS One 9 e109163 (2014)
PMID: 25275651

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169499 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase Mdm2 Chain: A
Molecule details ›
Chain: A
Length: 131 amino acids
Theoretical weight: 15.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q00987 (Residues: 6-102; Coverage: 20%)
Gene name: MDM2
Sequence domains: SWIB/MDM2 domain
Structure domains: SWIB/MDM2 domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 91%
Refinement method: distance geometry
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Polyphenols as Antitumor Agents Targeting Key Players in Cancer-Driving Signaling Pathways.
Cháirez-Ramírez et al. (2021)
2 more

1 mention without citation

Structural basis for inhibition of the MDM2:p53 interaction by an optimized MDM2-binding peptide selected with mRNA display.
Nagata et al. (2014)